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| 產品參數 | |||
|---|---|---|---|
| 品牌 | 菲恩生物 | ||
| 適用范圍 | 僅供科研使用 | ||
| 規格 | 100μg | ||
| 加工定制 | 否 | ||
| 驗證應用 | ELISA | ||
| WB | |||
| IHC | |||
| IP | |||
| 宿主 | 小鼠 | ||
| 適應種屬 | 人源 | ||
| 研究領域 | 新陳代謝，神經生物學，表觀遺傳學 | ||
| 可售賣地 | 全國 | ||
| 材質 | 液體 | ||
名稱：抗U2AF35抗體(anti-U2AF35 antibody)
貨號：FNab09138
適應種屬：人源
規格：100ug(微克)
狀態：液體
純化方法：蛋白A/G柱純化
純度：經sds-page分析，純度大于95
宿主：小鼠
亞型：IgG1
存儲：用添加了0.02疊氮化鈉和50甘油的pbs溶解，ph值7.3，可在-20℃下保存12個月（避免反復凍融）
免疫原：U2 small nuclear RNA auxiliary factor 1
免疫原別稱：DKFZp313J1712, FP793, RN, RNU2AF1, U2AF1, U2AF35, U2AFBP
分子量：38 kDa
驗證應用：ELISA, WB, IHC, IP
建議稀釋比例：WB: 1:500-1:5000; IHC: 1:20-1:200; IP: 1:200-1:2000
克隆性：單克隆
uniprot id：Q01081
背景介紹：U2 auxiliary factor (U2AF) , comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. U2 (RNU2) small nuclear RNA auxiliary factor 1 (U2AF1, synonyms: RN, FP793, U2AF35, U2AFBP, RNU2AF1) is the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. The U2AF1 gene is localized to chromosome 21q22.3, which is the critical region for three diseases, progressive myoclonus epilepsy, autoimmune polyglandular disease type 1, and one form of bipolar affective disorder。
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using FNab09138 (U2AF35 Antibody) at dilution of 1:50。
IP Result of anti-U2AF35 (IP:FNab09138, 3ug; Detection:FNab09138 1:500) with HeLa cells lysate 3000ug。
HeLa cells were subjected to SDS PAGE followed by western blot with FNab09138 (U2AF35 Antibody) at dilution of 1:1000。
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